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维生素B6对低蛋白日粮下断奶仔猪生长性能、肠道形态和基因表达的影响
发布单位:天津瑞孚农牧科技集团有限公司
查看次数：7401
时间：2020-03-04维生素B6（VB6）是生物体必需的功能性物质，在动物健康中不可或缺。然而，有关VB6和断奶仔猪肠道健康互作的研究基本没有。本试验目的在于研究低蛋白日粮（18%粗蛋白）下VB6对断奶仔猪生长性能、肠道健康、炎症因子和氨基酸转运载体mRNA表达的影响。
试验选用18头21日龄断奶杂交仔猪，初始重7.03±0.17kg，随机分至3个处理组，VB6添加量分别为0、4、7mg/kg。试验期14天。
试验结果表明处理对生长性能、腹泻率和生化指标没有显著影响。在空肠，VB6不影响肠道形态和Ki67阳性计数。4mg/kg VB6组降低了COX-2、IL-10和TGF-β的mRNA表达量（P＜0.05）。日粮添加7 mg/kg VB6提高了SLC7A1、SLC7A6、SLC16A14、SLC38A5的mRNA表达量（P＜0.05），4和7mg/kg VB6组降低了SLC36A1的mRNA表达量（P＜0.05）。在回肠，VB6不影响Ki67阳性计数，但是显著降低了绒毛面积（P＜0.05），并有降低绒毛高度的趋势（P=0.093）。日粮添加4mg/kg VB6显著提高了IL-1β、TNF-α、COX-2、IL-10和TGF-β的mRNA表达量（P＜0.05）。4和7mg/kg VB6组降低了SLC6A20、SLC7A1、SLC7A6、SLC16A14和SLC38A5的mRNA表达量（P＜0.05）。
结果表明日粮中添加VB6主要下调了空肠炎症因子的表达，上调了氨基酸转运载体的mRNA表达；而在回肠，上调了炎症因子的表达（4mg/kg），降低了氨基酸转运载体的mRNA的表达。这为断奶仔猪饲喂低蛋白日粮下肠道的发育提供了相关参考。
Effects of vitamin B6 on the growth performance, intestinal morphology, and gene expression in weaned piglets that are fed a low-protein diet
Vitamin B6 (VB6), which is an essential functional substance for biosome, plays an irreplaceable role in animal health. However, there are few studies that focus on the correlation between VB6 and intestinal health in weaned piglets. This study was conducted to investigate the effects of VB6 on the growth performance, intestinal morphology, and inflammatory cytokines and amino acid (AA) transporters mRNA expression in weaned piglets that are fed a low crude-protein (CP, 18%) diet. Eighteen crossbred piglets with initial body weights of 7.03 ± 0.17 kg (means ± SEM), weaned at 21-d age, were randomly assigned three diets with 0, 4, and 7 mg/kg VB6 supplementation, respectively. The experimental period lasted 14 days. Our results showed that there were no significant differences in growth performance, diarrhea rate, and biochemical parameters among the three treatments. In the jejunum, dietary VB6 supplementation did not affect the morphology and positive Ki67 counts. Dietary supplementation with 4 mg/kg VB6 decreased the mRNA expression of COX-2, IL-10, and TGF-β (P < 0.05). Dietary supplementation with 7 mg/kg VB6 increased the mRNA expression of SLC7A1, SLC7A6, SLC16A14, and SLC38A5 (P < 0.05) and 4 or 7 mg/kg VB6 decreased SLC36A1 mRNA expression (P < 0.05). In the ileum, VB6 supplementation did not affect positive Ki67 counts but significantly decreased villus area (P < 0.05) and tended to decrease villus height (P = 0.093). Dietary supplementation with 4 mg/kg VB6 had significantly increased the mRNA expression of IL-1β, TNF-α, COX-2, IL-10, and TGF-β (P < 0.05). Dietary supplementation with 4 or 7 mg/kg VB6 had significantly decreased SLC6A20, SLC7A1, SLC7A6, SLC16A14, and SLC38A5 mRNA expression (P < 0.05). These findings suggest that dietary supplementation of VB6 mainly down-regulated inflammatory cytokines and up-regulated AA transporters mRNA expression in jejunum, while up-regulated (4 mg/kg) inflammatory cytokines and down-regulated AA transporters mRNA expression in ileum, which may provide a reference for the intestinal development of weaned piglets that are fed a low-CP diet.
文章来源：猪营养国际论坛
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